Sensitive fluorescence analysis of aflatoxins using post-column UV derivatization on the Agilent 1260 Infinity LC

The analysis of aflatoxins in food matrices such as grain and nuts is an important task for the food industry to ensure product safety. This Application Note shows the analysis of the four major aflatoxins after sample preparation using an immunoaffinity column, post column UV derivatization, and fluorescence detection. We show that with this method the LOD and LOQ required from the regulatory agencies can be achieved easily. This method offers a less hazardous alternative compared to the well-established, post-column electrochemical derivatization method. Authors Yao Xiao and Rong An Agilent Technologies, Inc. Beijing, China Udo Huber Agilent Technologies, Inc. Waldbronn, Germany B2